Iryna B. Sorokulova, MS, PhD, DSCI

Professor of Microbiology, joined the College of Veterinary Medicine in 2002. She graduated from Taras Shevchenko National University (Kiev, Ukraine) with M.S. in Microbiology. She received her Ph.D. and D.Sc. Degrees in Microbiology from the Institute of Microbiology and Virology National Academy of Sciences of Ukraine. Dr. Sorokulova received professional training at Moscow State University (Russia), Bureau of Biologics (Ottawa, Canada), Massachusetts Biological Laboratories (Boston, MA, USA). She is the recipient of the Ukraine State Prize in Science and Technology.

Research Interests

Our research focuses on the development of new microbial approaches for detection and control of pathogens.

  • Therapeutic (probiotic) bacteria may provide a valuable alternative in the treatment of infections caused by pathogens. It was shown that probiotic bacteria can enhance intestinal barrier function. Probiotics are able to modulate signal transduction pathways and gene expression in epithelial and immune cells. Probiotic bacteria may improve both innate and adaptive immune responses. Probiotics were reported to regulate disturbed gut microflora. Our research interests are centered on Bacillus probiotic bacteria and their role in prevention of stress-related complications and treatment of infections due to multiresistant pathogens. Previously we have created several biotechnological products and saw them through all stages of development from bench top experiments to clinical practice. One of these products – probiotic Biosporin – is approved as a drug in Russia and Ukraine and is now on the market in these countries. Another product - recombinant probiotic Subalin - is approved as a drug in Ukraine.
  • Rapid detection of methycillin-resistant Staphylococcus aureus (MRSA) is of the key importance in prevention and prognosis of infections caused by antibiotic resistant S. aureus. Our approach in specific recognition and detection of MRSA  includes identification of bacteria together with confirmation of MRSA in real time. For this purpose, we used a newly isolated S.aureus bacteriophage with a wide range of hosts (including MRSA strains) together with monoclonal antibody against protein PBP 2a. PBP 2a is a cell wall protein responsible for antibiotic resistance of MRSA. Simultaneous recognition of Staphylococcus bacteria and PBP 2a protein increases specificity and reliability of MRSA detection.

Selected Publications

Sorokulova I, Watt J, Olsen E, Globa L, Moore T, Barbaree J, Vodyanoy V.  Natural biopolymer for preservation of microorganisms during sampling and storage, Journal of Microbiological Methods, 88, 1, 140-146, 2012.

Guntupalli R, Sorokulova I, Long R, Olsen E, Neely W, Vodyanoy V.  Phage Langmuir monolayers and Langmuir–Blodgett films, Colloids and Surfaces B: Biointerfaces 82; 182–189, 2011.

Sorokulova I, Krumnov A, Globa L, Vodyanoy V.  Efficient decomposition of shrimp shell waste using Bacillus cereus and Exiguobacterium acetylicum. Journal of Industrial Microbiology & Biotechnology, 36, (8): 1123-1126, 2009. 

Krumnov AA, Sorokulova IB, Olsen E, Globa L, Barbaree JM, Vodyanoy VJ.  Preservation of bacteria in natural polymers, Journal of Microbiological Methods, 78, 2, 189-194, 2009.

Kuzmicheva GA, Jayanna PK, Sorokulova IB, Petrenko VA.  Diversity and censoring of landscape phage libraries. Protein Eng. Des. Sel., 22, 1, 9-18, 2009.

Sorokulova IB.  Comparative study of Biosporin with other Bacillus probiotics. Rossiyskiy Vestnik perinatologii i Pediatrii (Russian Bulletin of Perinatology and Pediatrics) 53, 6, 41, 2008.

Sorokulova IB, Safronova LA, Vinogradov VN, Tyshkevich VN, Hilko TV, Starenkaya SYa, Lapa SV.  Correction of neonatal intestinal microflora disorders with Biosporin.  Rossiyskiy Vestnik Perinatologii I Pediatrii (Russian Bulletin of Perinatology and Pediatrics) 53, 5, 17-19, 2008. 

Kuzmicheva GA, Sorokulova IB, Jayanna PK, Petrenko VA.   Landscape phage libraries as a source of  bioselective nanomaterials. Nanotech 2008, 2008 v.2, 446-448, 2008.

Sorokulova IB, Krumnow AA, Pathirana S, Mandell AJ, Vodyanoy V.  Novel methods for storage stability and release of Bacillus spores. Biotechnology Progress. 24, (5):1147-1153, 2008.

Guntupalli R, Sorokulova I, Krumnow A, Pustovyy O, Olsen E, Vodyanoy V.  Real-time optical detection of methicillin-resistant Staphylococcus aureus using lytic phage probes. Biosens Bioelectron. 24(1):151-154, 2008.

Sorokulova IB, Pinchuk IV, Denayrolles M, Osipova IG, Huang JM, Cutting SM, Urdaci MC.  The safety of two Bacillus probiotic strains for human use. Dig Dis Sci. 53(4):954-963, 2008.

Sorokulova I.  Preclinical testing in the development of probiotics: a regulatory perspective with Bacillus strains as an example. Clinical Infectious Diseases, 46 (Suppl. 2), S92-S96, 2008.

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