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Differentiation

Determination of genetic polymorphisms of the amplified pathogen sequences allows typing of pathogens or detection of mutations associated with genetic diseases.

Melting point determination of the amplification products of Babesia gibsoni and Babesia canis.  The Babesia gibsoni/canis real-time PCR assay amplifies the target sequence of both species, but the sequences to which the green fluorescent donor probe attaches differ by 2 bases between B. gibsoni and B. canis.  These differences result in a Tm of 64.2°C for B. gibsoni and of 60.0 °C for B. canis and allow clear identification of each species by melting curve analysis in the same PCR.

© 2009 Auburn University College of Veterinary Medicine