Bruce F. Smith, V.M.D, Ph.D Dr. Smith is Associate Professor of Pathobiology , Scientist in the Scott-Ritchey Research Center and Director of the Scott-Ritchey Research Center's Molecular Medicine Program. He earned the V.M.D. degree from the University of Pennsylvania in 1988, where he was appointed a Kleberg Fellow in Medical Genetics. He received a PhD in Molecular Biology and Genetics from the University of Pennsylvania in 1993. His long range research interests are in gene therapy of inherited muscle diseases such as Duchenne muscular dystrophy, gene therapy of cancer and nucleic acid immunization.
334-844-5951 smithbf@vetmed.auburn.edu
Research Interests Dr. Smith’s research program in muscular dystrophies is currently focused on identifying novel canine models of these diseases. To date they have three unpublished models of X-linked Duchenne-like muscular dystrophy (DMD), as well as models of malignant hyperthermia and an autosomal recessive muscular dystrophy (ARMD). They are focused on identifying the gene defects in these models. They will then use them to develop gene therapy approaches to the muscular dystrophies, as well as DNA based testing programs to eliminate these diseases from the canine population. His research program on immunologic and gene therapy for cancer is part of a large collaborative program involving multiple investigators from Auburn University and the University of Alabama, Birmingham. Four project areas are now underway: Dendritic cell fusion vaccines in canine mammary cancer (Dr. R. Curtis Bird, PI) Conditionally replicative adenoviruses (CRADs) for canine osteosarcoma Dendritic cell targeted vaccines in canine melanoma Tumor cell targeted suicide gene therapy in canine lymphoma Selected Publications Books: Bird, R. C. and Smith, B. F., co-editors, Genetic Library Construction and Screening: Advanced Cloning Techniques and Applications, Springer-Verlag, 2002. Book Chapters: Samoilova, T. and Smith, B. In vivo phage display screening, in Genetic Library Construction and Screening: Advanced DNA Cloning Techniques and Applications, Springer-Verlag, 2002. Jiang, W. and Smith, B. Homemade TA cloning, in Genetic Library Construction and Screening: Advanced DNA Cloning Techniques and Applications, Springer-Verlag, 2002. Baker, H. J., Smith, B. F. , Martin, D. R. and Foureman, P. Molecular Diagnosis of the Feline Gangliosidoses: A Model for Elimination of Inherited Diseases in Pure Breeds. In August, J. Consultations in Feline Internal Medicine, Volume 4, W.B. Saunders, Co, Orlando, Florida, 2001. Refereed Journal Articles: Migone, F., DeInnocentes, P., Smith, B.F., and Bird, R.C. Alterations in CDK1 Expression and Localization Following Induction on a Spontaneous Canine Mammary Cancer Model. Submitted 2004. Hemminki, A., Kanerva, A., Kremer, E.J., Bauerschmitz G.J., Smith, B.F., Liu, B., Wang, M., Desomd, R.A., Keriel, A., Barnett, B, Baker, H.J., Siegal G.P., Curiel, D.T. A Canine Conditionally Replicating Adenovirus for Evaluating Oncolytic Virotherapy in a Syngeneic Animal Model. Molecular Therapy, 7(2)163-173, 2003. Smith, B.F., Migone, F.K., Cox, N.R., Baker, H.J. An In Utero Allotransplantation Model of Metastatic Breast Cancer in the Cat. In Vivo, 17(1), 2003. Jiang, W., Baker H.J., Smith, B.F. Mucosal Immunization with Helicobacter, CpG DNA and Cholera Toxin is Protective. Infection and Immunity, vol 71(1), January 2003. Whitley EM. Sellins KS. Ewald SJ. Smith BF. Yang S. Sartin EA. Wolfe LG. Canine mammary tumor cells transfected with B7-1 or B7-2 stimulate proliferation of peripheral blood mononuclear cells. Anticancer Research. 22(5):2567-74, 2002 Sep-Oct. Wang, C-Y. J., Giambrone, J. J., Smith, B.F., Detection of Duck Hepatitis B Virus DNA on Filter Paper by PCR and SyBr Green Dye-Based Quantitative PCR. Journal of Clinical Microbiology, 40(7): 2584-2590, 2002. Wang, C-Y. J., Giambrone, J. J., Smith, B.F., Comparison of cell culture systems for duck hepatitis B virus using SyBr green quantitative PCR. Journal of Virological Methods 106(2), 175-184, 2002. Wang, C-Y. J., Giambrone, J. J., Smith, B.F., Development of Viral Disinfectant Assays for Duck Hepatitis B Virus using Cell Culture / PCR. Journal of Virological Methods, 106(1), 39-50, 2002. Samoylov, A. M., Samoylova, T. I., Hartell, M. G., Pathirana, S. T., Smith, B. F., Vodyanoy, V. J. Recognition of Cell-Specific Binding of Phage Display Derived Peptides Using an Acoustic Wave Sensor. Biomolecular Engineering, 18(6):269-272, 2002. Wernette, C. M., Smith, B. F., *Barksdale, Z. M., Hecker, R., Baker, H. J. CpG Oligodexoynucleotides Stimulate Canine and Feline Immune Cell Proliferation. Veterinary Immunology and Immunopathology, 84:223-236, 2002. Nusbaum, K. E., Smith, B. F., DeInnocentes, P., Bird, R. C. DNA Vaccination of Channel Catfish Against Early and Late Transcripts of the Channel Catfish Herpesvirus (IHV-1). Veterinary Immunology and Immunopathology, 84:151-168, 2002.