Dr. Boudreaux is pictured with Gina, a Great Pyrenees with Glanzmann's thrombasthenia (GT), a type of inherited platelet disorder.

Mary K. Boudreaux, DVM, PhD

Dr. Mary K. Boudreaux, Professor in the Department of Pathobiology, earned the DVM degree in 1979 at Louisiana State University. She practiced veterinary medicine in California before beginning a clinical pathology residency program under Dr. Julia Blue at Cornell in 1981. She developed an interest in hemostasis and platelet pathophysiology and in 1986 earned a PhD degree in a program jointly sponsored by Cornell University and the Wadsworth Center for Laboratories and Research in Albany. Her co-directors for the research which evaluated regulatory control of cAMP phosphodiesterase in canine platelets were Dr. David O. Slauson, Dr. James Catalfamo, and Dr. W. J. Dodds. She joined the faculty at Auburn in 1986 and has developed research interests involving platelets.

334.844.2692
boudrmk@auburn.edu

Research Interests

My research interests include evaluating the role of platelets in disease pathogenesis and identification and characterization of congenital platelet disorders. Our platelet laboratory can isolate mammalian platelets from a variety of species for evaluation of platelet reactivity. We have developed a monoclonal antibody that detects a receptor-induced binding site (RIBS) on canine fibrinogen. The antibody has been useful in detection of platelet activation in dogs using flow cytometry and has also been useful in the characterization of congenital platelet disorders. As an extension of our interest in congenital platelet disorders we determined the normal gene sequences encoding platelet glycoproteins IIb and IIIa in dogs and horses. This work led to the discovery of the genetic basis for Glanzmann thrombasthenia (GT) in Great Pyrenees dogs and Otterhounds as well as the identification of 2 separate mutations causing GT in horses. During the late summer and fall of 2006 distinct mutations in the gene encoding calcium diacylglycerol guanine nucleotide exchange factor I (CalDAG-GEFI) were identified as causing thrombopathia in Basset hounds, Eskimo Spitz, Landseers of European Continental Type (ECT), and Simmental cattle. During the summer of 2007 a mutation in the gene encoding beta1-tubulin was found to correlate with the macrothrombocytopenia described in Cavalier King Charles Spaniels. Information on testing for GT in Great Pyrenees or Otterhounds, for CalDAG-GEFI thrombopathias in Basset hounds, Eskimo Spitz, or Landseers-ECT, and for congenital macrothrombocytopenia in Cavalier King Charles Spaniels can be found at the following links:

Great Pyrenees GT – Form for submission of blood samples for DNA testing.
Otterhound GT – Form for submission of blood samples for DNA testing.
Spitz Thrombopathia - Form for submission of blood samples for DNA testing.
Basset Hound Thrombopathia - Form for submission of blood samples for DNA testing.
Landseer Thrombopathia - Form for submission of blood samples for DNA testing.
Landseer Thrombopathia (Privacy Waived)- Form for submission of blood samples for DNA testing.
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Cavalier King Charles Spaniel Congenital Macrothrombocytopenia - Form for submission of blood samples for DNA testing

Other research interests have included identifying mutations associated with coagulation disorders in animals. In 2005, the gene sequence encoding coagulation factor IX in cats as well as the genetic basis for Hemophilia B in two domestic cats was reported.

Selected Publications

Davis B, Toivio-Kinnucan M, Schuller S, Boudreaux MK: Mutation in beta1-tubulin correlates with macrothrombocytopenia in Cavalier King Charles Spaniels. J Vet Int Med, accepted for publication, 2008.

Boudreaux MK, Schmutz S, French PS: Calcium diacylglycerol guanine nucleotide exchange factor I (CalDAG-GEFI) gene mutations in a thrombopathic Simmental calf. Vet Pathol, 44(6):932-935, 2007.

Boudreaux MK, Catalfamo JL, Klok M: Calcium Diacylglycerol Guanine Nucleotide Exchange Factor I gene mutations associated with loss of function in canine platelets. Translational Research 150:81-92, 2007.

Christopherson PW, van Santen VL, Livesey L, Boudreaux MK: A 10-base-pair deletion in the gene encoding platelet glycoprotein IIb associated with Glanzmann Thrombasthenia in a horse. J Vet Int Med 21(1):196-198, 2007.

Christopherson PW, Insalaco TA, van Santen VL, Livesey L, Bourne C, Boudreaux MK: Characterization of the cDNA encoding alpha IIb and beta 3 in normal horses and two horses with Glanzmann thrombasthenia. Vet Pathol, 43:78-82, 2006.

Goree M, Catalfamo JL, Aber S, Boudreaux MK: Characterization of the mutations causing Hemophilia B in two domestic cats. J Vet Intern Med, 19:200-204, 2005.

Livesey L, Christopherson P, Hammond A, Perkins J, Toivio-Kinnucan M, Insalaco, Boudreaux MK: Platelet dysfunction (Glanzmann’s Thrombasthenia) in horses. J Vet Int Med, 19:917-919, 2005.

Brdecka D, Adin C, Boudreaux MK, Crawford C, Randall S: Successful ovariectomy in a dog with Glanzmann Thrombasthenia. JAVMA, 224(11):1-3, 2004.

Niemeyer GP, Boudreaux MK, Goodman-Martin SA, Monroe CM, Wilcox DA, Lothrop CD Jr: Correction of a large animal model of type I Glanzmann’s thrombasthenia by nonmyeloblative bone marrow transplantation. Experimental Hematology 31:1357-1362, 2003.

Boudreaux MK: Platelets – Past, Present, and Future. Vet Clin Pathol 30(3):103, 2001. Guest Editorial.

Lipscomb DL, Bourne C, Boudreaux MK: Nucleotide sequence of the canine alpha IIb gene from platelet-derived cDNA. Am J Vet Res 62(9):1486-1492, 2001.

Boudreaux MK, Catalfamo JL: The molecular basis for Glanzmann’s thrombasthenia in Otterhounds. Am J Vet Res 62(11):1797-1804, 2001.

Boudreaux MK, Lipscomb DL: Clinical, Biochemical, and Molecular Aspects of Glanzmann’s Thrombasthenia in humans and dogs. Vet Pathol 38(3):249-260, 2001.

Lipscomb DL, Bourne C, Boudreaux MK: DNA sequence of the canine platelet β₃ gene from cDNA: Comparison of canine and mouse β₃ to segments that encode alloantigenic sites and functional domains of β₃ in human beings. J Lab Clin Med 134:313-321, 1999.

Boudreaux MK, Lipscomb DL, Catalfamo JL: Novel mutations within calcium binding domains of canine platelet GPIIb cause Type I Glanzmann’s thrombasthenia. Blood, 94(10) Supplement 1 (Part 2): 77b, 1999 (abstract).

Lipscomb D, Boudreaux MK, Bourne C: Comparison of the alpha IIb nucleotide sequence from platelet cDNA of thrombasthenic and normal dogs. Vet Pathol 36(5):482, 1999 (abstract).

Boudreaux MK, Catalfamo JL: Evaluation of the genes encoding for GP complex IIb-IIIa in Otterhounds with thrombasthenic thrombopathia. Vet Pathol 36(5):483, 1999 (abstract).

Boudreaux MK, Kvam K, Dillon AR, Bourne C, Scott M, Schwartz KA, Toivio-Kinnucan M: Type I Glanzmann's thrombasthenia in a Great Pyrenees dog. Vet Pathol 33:503-511, 1996.

Boudreaux MK, Panangala VS, Bourne C: A platelet activation-specific monoclonal antibody that recognizes a receptor-induced binding site on canine fibrinogen. Vet Pathol 33:419-427, 1996.

Boudreaux MK: Platelets and Coagulation: An update: Vet Clin North America Small Animal Practice 26(5):1065-1087, 1996.

Boudreaux MK, Crager C, Dillon AR, Stanz K, Toivio-Kinnucan M: Identification of an intrinsic platelet function defect in Spitz dogs. J Vet Int Med 8:93-98, 1994.