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What are the basics of your “molecular diagnostics”?

User: woffojl
Date: 7/24/2009 4:55 pm
Views: 370
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We first extract the nucleic acids from the samples, then use a technique called polymerase chain reaction (PCR) to detect the presence of pathogens.  PCR is a Nobel prize-winning molecular biology technique that amplifies certain nucleic acids exponentially, with the help of an enzyme called Taq polymerase.  With this technique, we can detect single copies of the target DNA and amplify it to billions of copies that we can easily detect and quantify.  For the RNA viruses (such as canine distemper virus, influenza virus and FIP virus), we do RT-PCRs which involves conversion of RNA to DNA followed by PCR.

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