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Molecular Diagnostics

We diagnose pathogens by quantitative detection of signature nucleic acid sequences with real-time polymerase chain reaction (PCR) methodology. Our approach to MICROBIOLOGICAL DIAGNOSTICS has been featured in eBIOforum Magazine.

Our lab is based on the application of PATENTED REAL-TIME PCR METHODS developed for research with intracellular Chlamydia bacteria.

Our real-time PCR delivers reliable RESULTS with rapid turnaround and results available WITHIN 24 HOURS after sample receipt.

At $70 PER ASSAY, critical PCR diagnoses are cost effective and translate rapidly - therefore effectively - into targeted therapy.

What's New...

With the rise in the cost of reagents and supplies, we are increasing the cost of our PCR  assays to $70 after five years of consistent pricing ($60). 

Our approach to FIP diagnosis has been featured in Veterinary Medicine. Please see the FIP assay for details.

Create your own CUSTOM PANEL at a discount  -  we now offer $10 multiple PCR discounts for every PCR after the first one submitted. The canine blood parasite panel for detection of Hepatozoon spp., Babesia spp., Anaplasma spp., Ehrlichia spp., and Borrelia spp. is the most popular combination requested by our clients. Please see our updated MD Submission Form.

All our real-time PCR assays are designed to detect single copies of nucleic acid targets in a large sample volume (equivalent to 0.1 mL of blood); and to recognize by use of fluorescent probes the relevant pathogens, differentiating them from closely related, but in the particular animal species less pathogenically relevant microbial organisms. Because of these strict quality standards, we typically offer the most sensitive and specific assays for these pathogens. Published investigations that use our assays continually confirm this and create NEW SCIENTIFIC INFORMATION that improves understanding and treatment of infectious diseases. Recent manuscripts from our laboratory were published in highly respected journals. These MANUSCRIPTS describe:

  • a new understanding of canine Hepatozoon spp. infection (Li et al., 2008; Allen et al., 2008);
  • improved diagnosis and treatment monitoring of Babesia spp. infection in dogs (Wang et al., 2010a);
  • unambiguous discrimination of FIV-vaccinated from FIV-infected cats with the assay for feline immunodeficiency virus, and identification of the subtype of the detected FIV strain (Wang et al., 2010b);
  • our Salmonella spp. PCR helped to investigate Salmonella enterica infections in leatherback turtle breeding grounds in the Caribbean (Dutton et al., 2013);
  • our Chlamydia spp., E. coli O157:H7, and pathogenic Salmonella spp., and Listeria spp. PCRs have been approved by the Food & Drug Administration (FDA) for monitoring of transgenically-produced biotechnology pharmaceutics;
  • our pathogenic Leptospira spp. PCR has been proven the most sensitive detection method for leptospira infection in dogs (Xu et al., 2014);
  • and with custom-developed real-time PCRs we have helped to analyze the impact of the Gulf oil spill on fish health (Crowe et al., 2014).
Auburn University | College of Veterinary Medicine | Auburn, Alabama 36849 | (334) 844-4546
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