Anaplasma phagocytophilum / platys
Anaplasma spp. are gram-negative nonmotile, coccoid to ellipsoid bacteria that infect many animal species and cause various diseases. They are obligate intracellular parasites and are usually transmitted through the bite of infected arthropods such as Ixodes scapularis and Rhipicephalus sanguineus. Anaplasma phagocytophilum, formerly known as Ehrlichia phagocytophila, infects neutrophils, and rarely eosinophils, of dogs, cats, ruminants, as well as humans and causes granulocytotropic anaplasmosis. Anaplasma platys, formerly known as Ehrlichia platys, causes thrombocytotropic anaplasmosis in dogs (Billeter et al., 2007).
Common clinical signs of canine anaplasmosis include high fever, lethargy, depression and polyarthitis. Neurologic signs (ataxia, seizures and neck pain) can also be seen. Anaplasma platys causes infectious canine cyclic thrombocytopenia (ICCT). Dogs infected with these bacteria will develop cyclic parasitemia and cyclic thrombocytopenia at 7- to 14-day intervals. A. platys formerly was not considered highly pathogenic, but recently cases of severe disease have been reported, similar in severity to Ehrlichia canis infections.
Standard Diagnostic Methods
Anaplasma infection can be detected via ELISA or IFA testing. Such serological testing can identify dogs with prior exposure but is prone to cross-reactivity and not very sensitive. Examination of blood smears can detect the pathogen but the sensitivity of this technique is very low. Molecular detection by PCR is very specific and sensitive, and hence is very useful to quickly identify this pathogen and confirm re-infection or carrier status (Martin et al., 2005).
The Molecular Diagnostics Laboratory at