Mary K. Boudreaux, DVM, PhD

• Great Pyrenees GT – Form for submission of blood samples for DNA testing.
• Otterhound GT – Form for submission of blood samples for DNA testing.
• Spitz Thrombopathia - Form for submission of blood samples for DNA testing.
• Basset Hound Thrombopathia - Form for submission of blood samples for DNA testing.
• Landseer Thrombopathia - Form for submission of blood samples for DNA testing.
• Landseer Thrombopathia (Privacy Waived)- Form for submission of blood samples for DNA testing.
• International Shipping Information
• Cavalier King Charles Spaniel Congenital Macrothrombocytopenia - Form for submission of blood samples for DNA testing
• Greater Swiss Mountain Dog Platelet Disorder - Form for submission of blood samples for DNA testing.
• LAD-I Variant or LAD III in German Shepherd Dogs - Form for submission of blood samples for DNA testing.

My research interests include evaluating the role of platelets in disease pathogenesis and identification and characterization of congenital platelet disorders.  Our platelet laboratory can isolate mammalian  platelets from a variety of species for evaluation of platelet reactivity.  We have developed a monoclonal antibody (CAP1) that detects a receptor-induced binding site (RIBS) on canine fibrinogen.  The antibody has been useful in detection of  platelet activation in dogs using flow cytometry and has also been useful  in the characterization of congenital platelet disorders.  Recently we have begun using flow cytometry and CAP1 to identify and characterize platelet disorders in animals that cannot be taken to Auburn University or other platelet testing facility.  These studies require 5 mls of CPDA-1 blood obtained from the affected animal and a normal control sent to my laboratory within 24 hours of blood collection.  For more information on these studies and prior to submission of samples, please contact Dr. Mary K Boudreaux (boudrmk@auburn.edu).  As part of our interest in congenital platelet disorders we determined the normal gene sequences encoding platelet glycoproteins IIb and IIIa in dogs and horses.  This work led to the discovery of the genetic basis for Glanzmann thrombasthenia (GT) in Great Pyrenees dogs and Otterhounds as well as the identification of 2 separate mutations causing GT in horses.  During the late summer and fall of 2006 distinct mutations in the gene encoding calcium diacylglycerol guanine nucleotide exchange factor I (CalDAG-GEFI) were identified as causing thrombopathia in Basset hounds, Eskimo Spitz, Landseers of European Continental Type (ECT), and Simmental cattle.  During the summer of 2007 a mutation in the gene encoding beta1-tubulin was found to correlate with the macrothrombocytopenia described in Cavalier King Charles Spaniels.   In 2009 a mutation in the gene encoding Kindlin-3, a signal transduction protein important in integrin activation in platelets and leukocytes, was identified in a German Shepherd dog.  The affected dog experienced life-long bleeding, high white cell counts, and susceptibility to infections.  This syndrome is referred to as LAD-I Variant or LAD-III.  Also in 2009 a mutation was identified in a gene encoding a platelet activating receptor in a Greater Swiss Mountain Dog.  This dog experienced prolonged bleeding following a routine spay.   

Information on testing for GT in Great Pyrenees or Otterhounds, for CalDAG-GEFI thrombopathias in  Basset hounds, Eskimo Spitz, or Landseers-ECT, for congenital macrothrombocytopenia in Cavalier King Charles Spaniels, for LAD-I Variant in German Shepherd dogs, and for a platelet disorder in Greater Swiss Mountain Dogs can be found below:

Platelet Disorder Diagnostic Testing

Other research interests have included identifying mutations associated with coagulation disorders in animals.  In 2005, the gene sequence encoding coagulation factor IX in cats as well as the genetic basis for Hemophilia B in two domestic cats was reported.  In 2009 a mutation was identified in the gene encoding factor VIII-C in a family of Boxers with Hemophilia A. 

Boudreaux MK, Wardrop KJ, Kiklevich V, Felsburg P, Snekvik K:  A mutation in the canine Kindlin-3 gene associated with increased bleeding risk and susceptibility to infections.  Thrombosis and Haemostasis  accepted for publication   103:2010. 

Davis B, Toivio-Kinnucan M, Schuller S, Boudreaux MK:  Mutation in beta1-tubulin correlates with macrothrombocytopenia in Cavalier King Charles Spaniels. J Vet Int Med  22:540-545, 2008.

Boudreaux MK, Schmutz S, French PS: Calcium diacylglycerol guanine nucleotide exchange factor I (CalDAG-GEFI) gene mutations in a thrombopathic Simmental calf. Vet Pathol 44(6):932-935, 2007.

Boudreaux MK, Catalfamo JL, Klok M:  Calcium Diacylglycerol Guanine Nucleotide Exchange Factor I gene mutations associated with loss of function in canine platelets.  Translational Research  150:81-92, 2007.

Christopherson PW, van Santen VL, Livesey L, Boudreaux MK:  A 10-base-pair deletion in the gene encoding platelet glycoprotein IIb associated with Glanzmann Thrombasthenia in a horse.    J Vet Int Med  21(1):196-198, 2007.

Christopherson PW, Insalaco TA, van Santen VL, Livesey L, Bourne C, Boudreaux MK:  Characterization of the cDNA encoding alpha IIb and beta 3 in normal horses and two horses with Glanzmann thrombasthenia.  Vet Pathol, 43:78-82, 2006.

Goree M, Catalfamo JL, Aber S, Boudreaux MK:  Characterization of the mutations causing Hemophilia B in two domestic cats.  J Vet Intern Med, 19:200-204, 2005. 

Livesey L, Christopherson P, Hammond A, Perkins J, Toivio-Kinnucan M, Insalaco, Boudreaux MK:  Platelet dysfunction (Glanzmann’s Thrombasthenia) in horses.  J Vet Int Med, 19:917-919, 2005.

Brdecka D, Adin C,  Boudreaux MK, Crawford C, Randall S:  Successful

ovariectomy in a dog with Glanzmann Thrombasthenia.  JAVMA, 224(11):1-3, 2004.

Niemeyer GP, Boudreaux MK, Goodman-Martin SA, Monroe CM, Wilcox DA, Lothrop CD Jr:  Correction of a large animal model of type I Glanzmann’s thrombasthenia by nonmyeloblative bone marrow transplantation.  Experimental Hematology  31:1357-1362, 2003.

Boudreaux MK:  Platelets – Past, Present, and Future.  Vet Clin Pathol  30(3):103, 2001.  Guest Editorial.

Lipscomb DL, Bourne C, Boudreaux MK:  Nucleotide sequence of the canine alpha IIb gene from platelet-derived cDNA.  Am J Vet Res 62(9):1486-1492, 2001.

Boudreaux MK, Catalfamo JL:  The molecular basis for Glanzmann’s thrombasthenia in Otterhounds.  Am J Vet Res  62(11):1797-1804, 2001.

Boudreaux MK, Lipscomb DL:  Clinical, Biochemical, and Molecular Aspects of Glanzmann’s Thrombasthenia in humans and dogs.  Vet Pathol  38(3):249-260, 2001.

Lipscomb DL, Bourne C, Boudreaux MK:  DNA sequence of the canine platelet β3 gene from cDNA:  Comparison of canine and mouse β3 to segments that encode alloantigenic sites and functional domains of β3 in human beings.  J Lab Clin Med  134:313-321, 1999.

Boudreaux MK, Lipscomb DL, Catalfamo JL:  Novel mutations within calcium binding domains of canine platelet GPIIb cause Type I Glanzmann’s thrombasthenia.  Blood, 94(10) Supplement 1 (Part 2): 77b, 1999 (abstract).

Lipscomb D, Boudreaux M, Bourne C:  Comparison of the alpha IIb nucleotide sequence from platelet cDNA of thrombasthenic and normal dogs.  Vet Pathol  36(5):482, 1999 (abstract).

Boudreaux MK, Catalfamo JL:  Evaluation of the genes encoding for GP complex IIb-IIIa in Otterhounds with thrombasthenic thrombopathia.  Vet Pathol 36(5):483, 1999 (abstract).

Boudreaux MK, Kvam K, Dillon AR, Bourne C, Scott M, Schwartz KA, Toivio-Kinnucan M: Type I Glanzmann's thrombasthenia in a Great Pyrenees dog. Vet Pathol 33:503-511, 1996.

Boudreaux MK, Panangala VS, Bourne C: A platelet activation-specific monoclonal antibody that recognizes a receptor-induced binding site on canine fibrinogen. Vet Pathol 33:419-427, 1996.

Boudreaux MK: Platelets and Coagulation: An update: Vet Clin North America Small Animal  Practice  26(5):1065-1087, 1996.

Boudreaux MK, Crager C, Dillon AR, Stanz K, Toivio-Kinnucan M: Identification of an intrinsic platelet function defect in Spitz dogs. J Vet Int Med 8:93-98, 1994.